Supplemental data to accompany:

**A bidirectional circuit switch reroutes pheromone signals in male and female brains**
Cell, Volume 155, Issue 7, 1610-1623, 19 December 2013 doi:10.1016/j.cell.2013.11.025
Johannes Kohl*, Aaron D. Ostrovsky*, Shahar Frechter, Gregory S.X.E. Jefferis

Data

The following data is available to accompany the publication

  • Tabular data underlying the figures is available through a dedicated R package, frulhns hosted on github.
    • The vignette for this package completely recreates the Supplemental statistics table for the paper.
    • The current version of the package requires the AnalysisSuite R codebase to be installed at a specific location (~/projects/AnalysisSuite, see github README) in order for the vignettes to be built from scratch.
    • Cached versions of the vignettes (frulhns v0.4.1) are available here:
  • Traced Neurons
  • 3D confocal image data (on a hard drive, on request to Greg)

Electrophysiological Analysis

Analysis and plotting of electrophysiological data initially acquired with Neuromatic/Nclamp was carried out in R, primarily using a custom R package, gphys. Although I would generally recommend using the latest version available via github, to install the specific package version associated with the manuscript, do as follows:

# Start R
 
# install (one time only)
install.packages("IgorR") # code to read Igor binary files (Greg)
install.packages("STAR") # code for Spike Train Analysis from Christophe Pouzat
# download gphys package from Jefferis lab website to a temporary file
download.file('http://flybrain.mrc-lmb.cam.ac.uk/si/frulhns/gphys-0.7-1.tar.gz',tf<-tempfile(fileext='.tgz'))
# install
install.packages(tf,repos=NULL,type='source')
# clean up temp file
unlink(tf)
 
library(gphys)
# get introductory help on package
?gphys
# get help on main plot function (including examples)
?PlotRasterFromSweeps 

note that this download includes full source code for the functions in the package along with documentation and examples.

Methods

Detailed methods are included in the manuscript supplemental information. Further information on these topics is available here


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