• Quantification of substrate and antibody penetration into fly brain
• Quantification of substrate and antibody penetration into mouse brain

Detailed methods are included in the manuscript methods and SI sections. Further information on these topics is available here

• Detailed cloning steps for fly stocks
• Fly brain staining protocol
• Mouse slice staining protocol
• Notes on substrates
• Recommended fly stocks
• Image Registration
• Morphological neuroanatomy in R aka AnalysisSuite

Please send any technical enquiries to tissuetagging@googlegroups.com to engage all authors of the paper. We will also use this list to update you about any improved stocks that we make. You can freely send email to that address but you must sign up here https://groups.google.com/forum/#!forum/tissuetagging to read existing discussions.

• There are some notes on the fly lines and reagents here that we recommend you read before contacting Greg to request any flies.
• Jefferis lab members can order these fly stocks (“PT stocks”) from Glynnis Johnson at Genetics (gh19@hermes.cam.ac.uk)

public preprint from http://biorxiv.org/ (includes a complete protocol for fly brain staining)

NEB SNAP/CLIP-tag

Promega Halo-tag

Active Motif TMP-tag (we used the covalent version)