Recommended fly stocks for chemical labeling
see full list chemical labeling fly stocks
membrane labeling
SNAP
- {myrSNAP}attP40 or {myrSNAP}attP2 better signal in neurites
- CD4-SNAP/CyO;CD4-SNAP/TM6b stock enriched in cell bodies
CLIP
- CD4-CLIP#1/CyO no myrCLIP available at the moment
Halo
- have used single attP40 and attP2 insertions which all worked; could try two insertions
TMP
- {myrTMP}attP40/CyO; {myrTMP}attP2 even when using this double insertion, signal is very weak (increase fixation to 40-60 min); only really useful for neuropil labeling
synaptic labeling
only SNAP and CLIP at the moment; in general, SNAP gives better signal-to-noise
pre
- SytCLIP#4/CyO or SytCLIP#6/TM3 these two P-element insertions have high expression levels (see list)
- SytSNAP#3/CyO or SytSNAP#2/TM3 these two P-element insertions have high expression levels (see list)
- CLIPsyb P-element insertions on 2 and 3, haven't thoroughly compared expression levels
- SNAPsyb P-element insertions on 2 and 3, haven't thoroughly compared expression levels
post
- TLN-CLIP#6/CyO or TLN-CLIP#9/TM3 these two P-element insertions have high expression levels (see list)
- TLN-SNAP#4/CyO or TLM-SNAP#5/TM3 these two P-element insertions have high expression levels (see list)
neuropil labeling
- brp-SNAP/CyO knock-in based on MiMIC insertion
- dlg-SNAP knock-in based on MiMIC insertion
- dlg1-SNAP product of P-element swap of protein trap line; weaker than the knock-in line
- dlg1-CLIP product of P-element swap of protein trap line
General Performance of tags
- labeling speed: SNAP > Halo > CLIP > TMP
- signal-to-noise: Halo > SNAP > CLIP > TMP
- signal intensity (with best available subtrate): SNAP,Halo > CLIP > TMP
take into consideration e.g. when using one tag for single-cell labeling and a second one for neuropil labeling