Supplemental data to accompany:

Ultra fast tissue staining using chemical tags Kohl et al. PNAS (2014)

and

Second-Generation Drosophila Chemical Tags: Sensitivity, Versatility, and Speed Sutcliffe et al. Genetics (2017)

Data

Image Analysis

Methods

Detailed methods are included in the manuscript methods and SI sections. Further information on these topics is available here

Information, Discussions & Enquiries

Please send any technical enquiries to tissuetagging@googlegroups.com to engage all authors of the paper. We will also use this list to update you about any improved stocks that we make. You can freely send email to that address but you must sign up here https://groups.google.com/forum/#!forum/tissuetagging to read existing discussions.

Resources

  • There are some notes on the fly lines and reagents here that we recommend you read before contacting Greg to request any flies.
  • Fly Stocks are freely available from the Bloomington stock centre
    • Jefferis lab members can order these fly stocks (“PT stocks”) from Glynnis Johnson at Genetics (gh19@hermes.cam.ac.uk)
  • Virus constructs have been deposited with Addgene (#60098 and 60099) and should be available by Dec 2014 at the latest:

public preprint from http://biorxiv.org/ (includes a complete protocol for fly brain staining)

NEB SNAP/CLIP-tag

Promega Halo-tag

Active Motif TMP-tag (we used the covalent version)


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