An introduction to NBLAST from Greg.
Very quick example of comparing 2 FlyCircuit neurons.
Detailed example of using a neuron mentioned in a recent paper on sleep by its FlyCircuit identifier to find structurally related neurons.
Example of searching for related neurons with a neuron id, showing the links from the results to virtualflybrain.org.
Searching for GMR GAL4 lines that label specific FlyCircuit neurons
Detailed example of making a tracing of a part of a group of neurons in registered image data downloaded from virtualflybrain.org.
Traced neurons can then be used as a query for NBLAST online (detailed run through).
Detailed example of making a skeletonised image from a lineage clone, with registered image data downloaded from virtualflybrain.org and using that for an NBLAST search.
Exploring Neuron Clusters
Starting from a search for a brain region (the AMMC) on the Virtual Fly Brain website we show how to look at structurally related neurons in 3D in your browser.
Assuming you have a neuron tracing, here is how to find the most similar neuron in a database in R using the nat.nblast package.
Here is an example of how to run code that generates some of the figures in our paper. The code for all figures is available on our lab github repo.
We recommend Chrome for NBLAST online, but you can also use Firefox or Safari. For Safari you must activate WebGL: Here’s how.