Parts
- There is a also a useful supported parts list for ScanImage software
Laser
Coherent Chameleon Vision (A, below). Specs are here
Core Microscope
Sutter MOM (Moveable Objective Microscope).
- Fluorescence path
- Point Grey Grasshopper CCD camera (A)
- Olympus tube lens (B)
- Olympus epi-fluorescence path (from BX51) (C)
- Mirrors of MOM core (L)
- Objective lens (variable) (E)
- Two-photon detection path
- Objective lens (E)
- Dichroic mirror in MOM core (L)
- Hamamatsu PMTs (and collection optics) (F)
- Hamamatsu pre-amps (G)
- Other
- PMT Power supply (H)
- iMac (O)
- Power meters (J)
- Power sensor (K)
- Stage (D)
- Cage light (M)
- Cage (N)
- Thorlabs cubes (for controlling half-wave plate, fluorescence LED, and shutter (I)
- Odour presentation manifold (P)
- Aaron's junk (Q)
Scan System
Fast scan 6mm mirrors (Cambridge 6215) and scanner driver electronics (includes two Cambridge Technologies 671XX-HP Micromax scanner driver boards)
PMTs
Hamamatsu H7422 P40-MOD unprotected GAsP PMTs (Hamamatsu part number, H10770PB-40 SEL) and Hamamatsu C3719 pre amps. The power supply is labeled “Sutter PS-2” but it looks suspiciously like two Hamamatsu model C3830 supplies piled into a Sutter box. I can't find any info on the PS-2, but the Hamatsu specs are here or here. This is just a guess on my part, so if you are really fussed about these specs, it would probably be best to contact Sutter.
Other Parts
- Filters/dichroic for detection
- Chroma
- Table
- Newport Smart Table UT2 with IQ Damping Technology
- Attenuation
- Thorlabs (WPH05M) rotating half-wave plate (B) and fixed (GL10-B) Glan-Laser polariser (D) to control beam intensity
- Non-polarising (Thorlabs CM1-BS014) 50:50 beam splitter (F) to divide the beam
- Variable (Thorlabs BE02-05-B) beam expander (I) to control objective (over/under) filling
- Power measurement
- J beam sampler (reflected portion is angle dependent - currently around 3%)
- L Power meter
- note that you need to re-calibrate the linear relationship between power meter reading and power at the objective back aperture if you
- knock/realign J
- put telescope in
- Shutters
- Thorlabs SH05 (G) This shutter is normally opened by scanImage during a run. Additionally, it is connected to an interlock on the shroud door, forced closed when the door is open.
- Beam steering on table
- Thorlabs mirrors (E, H)
- Beam trap
- Thorlabs BT610/M (C, K)
- PCs for control
- Lenovo Thinkstation with NI DAQ board (NI 6110E) for ScanImage operation
- iMac with Micromanager
Objectives
- Zeiss 20x 1.0 NA M27 (70 mm parfocal, 75 also avail w more DIC)
- Olympus 20x 1.0 NA 25 diam
- Olympus 40x 0.8 NA (RMS mount)
- Olympus 25x 1.05 NA (? mount)
Software
- Svoboda ScanImage and Ephus
Filters for PMTs
Alex set up the filter cube for the PMTs as below:
RED CHANNEL *-------------* | HQ605/75 \/ | *---*-------------* G | |\* R | E | \* E | T | \* E | 5 | \* N | 2 | \* | 5 | \* C | / | \* H | 7 | \* A | 0 | \* N | | \* N | | \* E | > | \* L | | \* *---*
The filters are by Chroma and the arrows point towards the coated side.