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si:tagging:start [2014/11/13 20:15] – jkohlsi:tagging:start [2026/11/08 10:57] (current) – Replace raw-HTML Addgene button with wiki syntax jefferis
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 **Ultra fast tissue staining using chemical tags** **Ultra fast tissue staining using chemical tags**
 [[http://www.pnas.org/content/111/36/E3805.full|Kohl et al. PNAS (2014)]] [[http://www.pnas.org/content/111/36/E3805.full|Kohl et al. PNAS (2014)]]
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 +and
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 +**Second-Generation Drosophila Chemical Tags: Sensitivity, Versatility, and Speed**
 +[[http://www.genetics.org/content/205/4/1399.long|Sutcliffe et al. Genetics (2017)]]
 +
  
 ====== Data ====== ====== Data ======
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 =====Resources===== =====Resources=====
   * There are some notes on the fly lines and reagents {{:si:tagging:tissuetaggingflies.pdf|here}} that we recommend you read before contacting Greg to request any flies.    * There are some notes on the fly lines and reagents {{:si:tagging:tissuetaggingflies.pdf|here}} that we recommend you read before contacting Greg to request any flies. 
-  * Jefferis lab members can order these fly stocks ("PT stocks") from Glynnis Johnson at Genetics (gh19@hermes.cam.ac.uk)+  * Fly Stocks are freely available from the [[http://flystocks.bio.indiana.edu/Browse/misc-browse/chemtag.php|Bloomington stock centre]] 
 +    * Jefferis lab members can order these fly stocks ("PT stocks") from Glynnis Johnson at Genetics (gh19@hermes.cam.ac.uk) 
 +  * Virus constructs have been deposited with Addgene (#60098 and 60099) and should be available by Dec 2014 at the latest: 
 +[[https://www.addgene.org/Gregory_Jefferis|{{ https://www.addgene.org/static/images/plasmid_request_button_no_pi.png?nolink&0x140 |Link to Gregory Jefferis Lab Plasmids}}]]
  
 +[[https://www.addgene.org/Gregory_Jefferis|Find Gregory Jefferis Lab Plasmids]] at Addgene
 ===== Links ===== ===== Links =====
 [[http://biorxiv.org/content/early/2014/05/19/005298|public preprint]] from http://biorxiv.org/ (includes a complete protocol for fly brain staining) [[http://biorxiv.org/content/early/2014/05/19/005298|public preprint]] from http://biorxiv.org/ (includes a complete protocol for fly brain staining)

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