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si:tagging:start [2014/11/13 20:11] – jkohlsi:tagging:start [2026/11/08 10:57] (current) – Replace raw-HTML Addgene button with wiki syntax jefferis
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 **Ultra fast tissue staining using chemical tags** **Ultra fast tissue staining using chemical tags**
 [[http://www.pnas.org/content/111/36/E3805.full|Kohl et al. PNAS (2014)]] [[http://www.pnas.org/content/111/36/E3805.full|Kohl et al. PNAS (2014)]]
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 +and
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 +**Second-Generation Drosophila Chemical Tags: Sensitivity, Versatility, and Speed**
 +[[http://www.genetics.org/content/205/4/1399.long|Sutcliffe et al. Genetics (2017)]]
 +
  
 ====== Data ====== ====== Data ======
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 Detailed methods are included in the manuscript methods and SI sections. Further information on these topics is available here Detailed methods are included in the manuscript methods and SI sections. Further information on these topics is available here
   * [[tagging2014fly-cloning|Detailed cloning steps for fly stocks]]   * [[tagging2014fly-cloning|Detailed cloning steps for fly stocks]]
-  * [[tagging2014virus-cloning|Detailed cloning steps for virus constructs]] 
   * [[tagging2014flystain |Fly brain staining protocol]]   * [[tagging2014flystain |Fly brain staining protocol]]
   * [[tagging2014mousestain |Mouse slice staining protocol]]   * [[tagging2014mousestain |Mouse slice staining protocol]]
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 =====Resources===== =====Resources=====
   * There are some notes on the fly lines and reagents {{:si:tagging:tissuetaggingflies.pdf|here}} that we recommend you read before contacting Greg to request any flies.    * There are some notes on the fly lines and reagents {{:si:tagging:tissuetaggingflies.pdf|here}} that we recommend you read before contacting Greg to request any flies. 
-  * Jefferis lab members can order these fly stocks ("PT stocks") from Glynnis Johnson at Genetics (gh19@hermes.cam.ac.uk)+  * Fly Stocks are freely available from the [[http://flystocks.bio.indiana.edu/Browse/misc-browse/chemtag.php|Bloomington stock centre]] 
 +    * Jefferis lab members can order these fly stocks ("PT stocks") from Glynnis Johnson at Genetics (gh19@hermes.cam.ac.uk) 
 +  * Virus constructs have been deposited with Addgene (#60098 and 60099) and should be available by Dec 2014 at the latest: 
 +[[https://www.addgene.org/Gregory_Jefferis|{{ https://www.addgene.org/static/images/plasmid_request_button_no_pi.png?nolink&0x140 |Link to Gregory Jefferis Lab Plasmids}}]]
  
 +[[https://www.addgene.org/Gregory_Jefferis|Find Gregory Jefferis Lab Plasmids]] at Addgene
 ===== Links ===== ===== Links =====
 [[http://biorxiv.org/content/early/2014/05/19/005298|public preprint]] from http://biorxiv.org/ (includes a complete protocol for fly brain staining) [[http://biorxiv.org/content/early/2014/05/19/005298|public preprint]] from http://biorxiv.org/ (includes a complete protocol for fly brain staining)

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