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si:tagging:start [2014/06/10 12:04] – [Ultra fast tissue staining with chemical tags] jefferissi:tagging:start [2026/11/08 10:57] (current) – Replace raw-HTML Addgene button with wiki syntax jefferis
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-====== Ultra fast tissue staining with chemical tags ======+Supplemental data to accompany:
  
-This page provides pointers to some additional resources for a paper, presently in the final stages of preparation.+**Ultra fast tissue staining using chemical tags** 
 +[[http://www.pnas.org/content/111/36/E3805.full|Kohl et al. PNAS (2014)]]
  
-You can download a [[http://biorxiv.org/content/early/2014/05/19/005298|public preprint]] from http://biorxiv.org/, which includes protocols and other resources.+and
  
-There are also some notes on the fly lines and reagents {{:si:tagging:tissuetaggingflies.pdf|here}} that we recommend you read before contacting Greg to request any flies. +**Second-Generation Drosophila Chemical Tags: Sensitivity, Versatility, and Speed** 
 +[[http://www.genetics.org/content/205/4/1399.long|Sutcliffe et al. Genetics (2017)]]
  
-Please send any technical enquiries to tissuetagging@googlegroups.com to engage all authors of the paper. You can freely send email to that address but you must sign up here https://groups.google.com/forum/#!forum/tissuetagging to read existing discussions. 
  
 +====== Data ======
 +
 +
 +====== Image Analysis ======
 +  * [[fly image analysis | Quantification of substrate and antibody penetration into fly brain]]
 +  * [[fiber analysis in mouse brain slices | Quantification of substrate and antibody penetration into mouse brain]]
 +====== Methods ======
 +Detailed methods are included in the manuscript methods and SI sections. Further information on these topics is available here
 +  * [[tagging2014fly-cloning|Detailed cloning steps for fly stocks]]
 +  * [[tagging2014flystain |Fly brain staining protocol]]
 +  * [[tagging2014mousestain |Mouse slice staining protocol]]
 +  * [[tagging2014substrates|Notes on substrates]]
 +  * [[tagging2014recflies|Recommended fly stocks]]
 +  * [[warping_manual:start|Image Registration]]
 +  * [[https://github.com/jefferis/AnalysisSuite|Morphological neuroanatomy in R]] aka AnalysisSuite
 +
 +=====Information, Discussions & Enquiries=====
 +Please send any technical enquiries to tissuetagging@googlegroups.com to engage all authors of the paper. We will also use this list to update you about any improved stocks that we make.
 +You can freely send email to that address but you must sign up here https://groups.google.com/forum/#!forum/tissuetagging to read existing discussions.
 +=====Resources=====
 +  * There are some notes on the fly lines and reagents {{:si:tagging:tissuetaggingflies.pdf|here}} that we recommend you read before contacting Greg to request any flies. 
 +  * Fly Stocks are freely available from the [[http://flystocks.bio.indiana.edu/Browse/misc-browse/chemtag.php|Bloomington stock centre]]
 +    * Jefferis lab members can order these fly stocks ("PT stocks") from Glynnis Johnson at Genetics (gh19@hermes.cam.ac.uk)
 +  * Virus constructs have been deposited with Addgene (#60098 and 60099) and should be available by Dec 2014 at the latest:
 +[[https://www.addgene.org/Gregory_Jefferis|{{ https://www.addgene.org/static/images/plasmid_request_button_no_pi.png?nolink&0x140 |Link to Gregory Jefferis Lab Plasmids}}]]
 +
 +[[https://www.addgene.org/Gregory_Jefferis|Find Gregory Jefferis Lab Plasmids]] at Addgene
 ===== Links ===== ===== Links =====
 +[[http://biorxiv.org/content/early/2014/05/19/005298|public preprint]] from http://biorxiv.org/ (includes a complete protocol for fly brain staining)
 +
 +[[https://www.neb.com/tools-and-resources/feature-articles/snap-tag-technologies-novel-tools-to-study-protein-function|NEB SNAP/CLIP-tag]]
  
-[[https://www.neb.com/tools-and-resources/feature-articles/snap-tag-technologies-novel-tools-to-study-protein-function|NEB SNAP/CLIP]]+[[http://www.promega.co.uk/products/pm/halotag-technology/halotag-technology/|Promega Halo-tag]]
  
-[[http://www.promega.co.uk/products/pm/halotag-technology/halotag-technology/|Promega Halo Tag]]+[[http://www.activemotif.com/catalog/122/ligandlink-universal-protein-labeling|Active Motif TMP-tag]] (we used the covalent version)

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