200 µm coronal vibratome brain slices of animals injected with AAV-myrSNAP were incubated with 1 µM BG-549 for 30 min, 1 h, 6 h or 12 h. Confocal stacks were resliced and individual neurites that projected through the whole depth of the stack (n = 9–11 per condition) were traced using the ImageJ Simple Neurite Tracker tool; the tracing paths were converted to a binary mask by thresholding the result of the “Fill Out” option of Simple Neurite Tracer. Voxel intensity data were then fit as a function of z position with a gam smoothing line in R using the default options of ggplot2::stat_smooth. Antibody penetration into mouse brain sections was assessed by incubating coronal vibratome slices of the brains of PV-Cre animals injected with AAV-myrSNAP-CON with monoclonal mouse T49 anti-Tau antibody (1:2,000) for 30 min, 1 h, 6 h or 12 h, followed by washing and 2 d incubation with secondary antibody (anti-mouse Alexa 647, 1:1,000). Data were quantified by reslicing confocal stacks in ImageJ, making a maximum intensity projection and then using R to plot the mean image intensity at each z location, with a loess smoothing line using the default options of ggplot2::stat_smooth.