https://flybrain.mrc-lmb.cam.ac.uk/dokuwiki/ 2026-05-14T19:02:53+00:00 https://flybrain.mrc-lmb.cam.ac.uk/dokuwiki/ https://flybrain.mrc-lmb.cam.ac.uk/dokuwiki/lib/tpl/monobook/images/favicon.ico text/html 2013-12-10T11:59:45+00:00 Anonymous (anonymous@undisclosed.example.com) https://flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?id=protocols:image_cells_using_the_strobelight_micromanager_plugin&rev=1386676785&do=diff StrobeLight Plugin A slightly more detailed description of the plugin can be found here Installation If you are starting with a stock version of Micromanager, you will need to install both the modified dc1394 adapter and the java plugin. All files can be found in /Volumes/JData/JPeople/Common/CommonCode/StrobeLightFiles. You can download them temporarily and put them anywhere you want, but for the following instructions to make sense, just copy the whole StrobeLightFiles folder to your deskto… text/html 2013-12-10T11:59:45+00:00 Anonymous (anonymous@undisclosed.example.com) https://flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?id=protocols:janelia_flylight_data_dump&rev=1386676785&do=diff Accessing Lab Copy * The lab copy is on the Synology 12 drive NAS. * We have had problems with dropped iscsi connections to that drive * when a connection drops, a computer reboot seems to be required * so it is not currently shared by GJAC text/html 2013-12-10T11:59:45+00:00 Anonymous (anonymous@undisclosed.example.com) https://flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?id=protocols:saline_recipes&rev=1386676785&do=diff Phosphate Buffer (0.1M PB) For dissection before fixation. Amount Final Concentration NaH2PO4·H2O 4.3633 g .032 M Na2HPO4 anhydrous 9.705 g .068 M H2O 1 l Phosphate Buffer (0.125M PB) For diluting 20% formaldehyde to make a 4% fix solution which has 0.1M phosphate buffer.