Every image in our dataset was registered against a template brain to bring them into a standard coordinate system resulting in a reformatted image. The reformatted images were then extracted using different binary masks according to which fru+ MARCM clones were present. So each image could potentially result in e.g. 10 different extracted images if it contained 10 completely isolated MARCM clones.

Every original image has a unique identifier of the form:

This can be decomposed into

• SlideKey (eg SAEJ)
• BrainNumber (eg 26)

to make SAEJ26 which refers to a single brain. Each image of this brain is then sequentially numbered

• i.e. SAEJ26-1 is the first image for brain SAEJ26.