In additional to supplemental data available via the Current Biology website, we are providing two large collections of image data:
- 3Gb of key image data (available on the Web)
- >1Tb of comprehensive image data including all raw and processed confocal stacks (available by external hard drive)
Web Supplement
You can access the web supplemental data here:
http://flybrain.mrc-lmb.cam.ac.uk/CacheroOstrovsky/Data/
The directory hierarchy that you will see includes:
- Standard Template Brains and Ventral Nerve Cords
- Brain
- VNC
- Single full sized image stacks of each fruitless neuroblast clone
- Brain
- Female
- Male
- VNC
- Male
- Female
- Resampled image stacks of each clone (suitable for use with Amira)
- Brain
- Female
- Male
- VNC
- Male
- Female
- Mask files used to extract clones from registered images
- Brain
- VNC
- Registration files used for Deformation Based Morphometry
- femaleControl
- fruMFemales
- maleControl
- traFemales
- traMales
- Doublesex/fruitless double immunolabelled stacks
- female
- male
Hard drive Supplement
This includes all raw Brain and VNC data used in our study (1.05 Tb).
There are Brain and VNC top level directories each containing the following :
- RawLSMs : MARCM Confocal stacks from our Zeiss LSM 710 (984,364 LSM files)
- Registrations : Registration output folders including settings and logs (737,299 CMTK .list folders)
- SuccessfullyRegistered : Tissues after registration - brains that did not register well are excluded (1533, 724 PIC files)
- ExtractedImages : Masked images generated for each clone present in each image (908, 241 NRRD files)
- TwinSpotBrains: Brains labelled with twin spot MARCM so that clones are either in red or green but not both (270,0 LSM files)
- A csv spreadsheet ExportedImageRecords.txt which includes additional information about the images used
(numbers in parentheses indicate the number of Brain and VNC images respectively of each type)
