The servo controller module inside the E665 control unit can be tuned to move the PI-726 piezo objective holder as fast as possible. 6ms should be achievable for 10µm steps with the Zeiss 20x objective.

  • Unplug
  • Take the lid off (6 screws side and rear)
  • Plug back in
  • Identify servo module (see manual)
  • Identify P1-P4 and P6 potentiometers
  • Choose a step size on the large end of what you will use for a single step (we did 10µm)
  • Have Igor give a whole load of pulses at that step size
  • Change P3 and P2 to make it faster (these are integration time and gain of feedback loop)
  • If you can't get any faster then change P1 Slew rate limiter to allow faster movement
  • Try to get something as square as possible and be prepared to have a very slight overshoot at the start of the step (100-200nm may well be irrelevant)
  • Adjust P6 (it may need quite a few turns) to get step size to be exactly 10µm again.

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