The servo controller module inside the E665 control unit can be tuned to move the PI-726 piezo objective holder as fast as possible. 6ms should be achievable for 10µm steps with the Zeiss 20x objective.
- Unplug
- Take the lid off (6 screws side and rear)
- Plug back in
- Identify servo module (see manual)
- Identify P1-P4 and P6 potentiometers
- Choose a step size on the large end of what you will use for a single step (we did 10µm)
- Have Igor give a whole load of pulses at that step size
- Change P3 and P2 to make it faster (these are integration time and gain of feedback loop)
- If you can't get any faster then change P1 Slew rate limiter to allow faster movement
- Try to get something as square as possible and be prepared to have a very slight overshoot at the start of the step (100-200nm may well be irrelevant)
- Adjust P6 (it may need quite a few turns) to get step size to be exactly 10µm again.