We currently have one multiphoton system based around a Sutter MOM core assembled and accessorised by Alex. Software control is by ScanImage running under Matlab on a PC, communicating via NI DAQ board. See:

• Alex's two-photon project page for details for of the hardware setup of 2P number 1 (The 2P furthest from the confocal).
• NI Board Connections
• objective mount
• Problems
• Piezo objective
• Spatial Calibration
• Sample view/movement
• MP285
• ChRimson LED

Use these logs to record system history/modification. They may well point to other pages etc.

• 2P1 Log
• 2P2 Log
• 2P2 TODO
• ScanImage notes about installation/versions

• Basic use of 2p system (copied over from PC Desktop in first instance)
• Detailed step by step use of 2p system
• Aligning the 2p laser
• ScanImage Docs and Matlab Compatibility matrix
• Retune scan mirror controllers
• Tune Piezo e.g to make it as fast as possible for specific objective/step size
• Remove detector head
• Config Files
• Recenter MP285 axes
• Set MP285 Home
• Clean the Filters (from Semrock website, includes the Brightline multiphoton 750 SP blocker)
• Excitation Wavelengths for dyes and fluorescent proteins
• Change Coolant for Laser

See also the general lab Safety page

• 2P Risk Assessment
• Class IV Laser Safety RA 14_sutter_movable_objective_multiphoton_microscope_ra_01082019.docx
• Standard Operating Procedures sutter_movable_objective_microscope_sop_normal_and_alignment_use.pdf

• We have a 3 year extended warranty with Coherent until 18th March 2014 - details in this pdf
• Contact at Coherent is Mike Shearer (mike.shearer@coherent.com - Tel: 01353 65 88 54)