====== Hardware ======
We currently have one multiphoton system based around a Sutter MOM core assembled and accessorised by Alex. This has been modified, moved, and upgraded by Bruno Pichler's company (INSS) and Istvan.  Software control is by ScanImage running under Matlab on a PC, communicating via NI DAQ board.  See:
  * [[Hardware details after move to 3N390]]
  * [[alex:projects:multiphoton:start|Alex's two-photon project page]] - obsolete.
  * [[NI Board Connections]]
  * [[objective mount]]
  * [[Problems]]
  * [[Piezo objective]]
  * [[Spatial Calibration]]
  * [[Sample view/movement]]
  * [[MP285]]
  * [[ChRimson LED]]
====== Logs ======
Use these logs to record system history/modification. They may well point to other pages etc.
  * [[2P1 Log]]
  * [[2P2 Log]]
  * [[2P2 TODO]]
  * [[ScanImage]] notes about installation/versions
====== How Tos ======
  * [[Basic use of 2p system]] (copied over from PC Desktop in first instance)
  * [[imaging_2p|Detailed step by step use of 2p system]]
  * [[protocols:Aligning the 2p laser]]
  * [[http://research.janelia.org/wiki/display/ephus/ScanImage%2C+Ephus%2C+and+other+DAQ+software|ScanImage Docs]] and [[http://www.neuroptikon.org/projects/display/ephus/ScanImage+Matlab+and+NI+Version+Compatibility|Matlab Compatibility matrix]]
  * [[Retune scan mirror controllers]]
  * [[Tune Piezo]] e.g to make it as fast as possible for specific objective/step size
  * [[Remove detector head]]
  * [[Config Files]]
  * [[Recenter MP285 axes]]
  * [[Set MP285 Home]]
  * [[http://www.semrock.com/cleaning-optical-filters.aspx|Clean the Filters]] (from Semrock website, includes the [[http://www.semrock.com/brightline-multiphoton-fluorescence-filters.aspx|Brightline multiphoton]] 750 SP blocker)
  * [[aaron:projects:excitation_of_alexa_dyes_and_fps|Excitation Wavelengths for dyes and fluorescent proteins]]
  * [[Change Coolant for Laser]]
  * [[2P Dissections]]
  * [[https://www.biorxiv.org/content/10.1101/2024.01.23.576417v1|Packer Multiphoton Testing Protocols]]
====== Safety ======

See also the general lab [[safety:start|Safety page]]

  * [[2P Risk Assessment]]
  * Class IV Laser Safety RA {{ :equipment:multiphoton:ra_sutter_movable_objective_multiphoton_microscope.docx |}}
  * Standard Operating Procedures {{ :equipment:multiphoton:sutter_movable_objective_microscope_sop_normal_and_alignment_use.pdf |}}
====== Maintenance ======
  * We have a 3 year extended warranty with Coherent until 18th March 2014 - {{:equipment:multiphoton:coherentmaintenance2011-2014.pdf|details in this pdf}}
  *  Contact at Coherent is Mike Shearer (mike.shearer@coherent.com - Tel: 01353 65 88 54)