=== Navigation === Considering the co-ordinates of the image to have an origin in the top left, movements map as: ^ Image direction ^ Direction on MP285 ^ Label on hand controller ^ | -y | -x | North | | -x | +y | Right | | Into specimen | +z | Down | As we have rotated the camera and flipped the scan direction in ScanImage, these directions are now the same on both the wide-field view in MicroManager on the iMac, and the view in ScanImage on the PC. === Switching from wide-field to 2-photon view === It necessary to move up ~85 microns, in Z, in order to view the same object in 2-photon mode as has been seen in wide-field mode. This corresponds to ~3/4 of a turn of the dial on the hand controller when in fine mode.