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equipment:multiphoton:start [2017/01/10 15:04] – [Maintenance] edonaequipment:multiphoton:start [2024/04/18 05:45] (current) – [How Tos] jefferis
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 ====== Hardware ====== ====== Hardware ======
-We currently have one multiphoton system based around a Sutter MOM core assembled and accessorised by Alex.  Software control is by ScanImage running under Matlab on a PC, communicating via NI DAQ board.  See: +We currently have one multiphoton system based around a Sutter MOM core assembled and accessorised by Alex. This has been modified, moved, and upgraded by Bruno Pichler's company (INSS) and Istvan.  Software control is by ScanImage running under Matlab on a PC, communicating via NI DAQ board.  See: 
-  * [[alex:projects:multiphoton:start|Alex's two-photon project page]] for details for of the hardware setup of 2P number 1 (The 2P furthest from the confocal).+  * [[Hardware details after move to 3N390]] 
 +  * [[alex:projects:multiphoton:start|Alex's two-photon project page]] - obsolete.
   * [[NI Board Connections]]   * [[NI Board Connections]]
   * [[objective mount]]   * [[objective mount]]
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   * [[ScanImage]] notes about installation/versions   * [[ScanImage]] notes about installation/versions
 ====== How Tos ====== ====== How Tos ======
-  * [[http://research.janelia.org/wiki/display/ephus/ScanImage%2C+Ephus%2C+and+other+DAQ+software|ScanImage Docs]] and [[http://www.neuroptikon.org/projects/display/ephus/ScanImage+Matlab+and+NI+Version+Compatibility|Matlab Compatibility matrix]] 
   * [[Basic use of 2p system]] (copied over from PC Desktop in first instance)   * [[Basic use of 2p system]] (copied over from PC Desktop in first instance)
 +  * [[imaging_2p|Detailed step by step use of 2p system]]
   * [[protocols:Aligning the 2p laser]]   * [[protocols:Aligning the 2p laser]]
 +  * [[http://research.janelia.org/wiki/display/ephus/ScanImage%2C+Ephus%2C+and+other+DAQ+software|ScanImage Docs]] and [[http://www.neuroptikon.org/projects/display/ephus/ScanImage+Matlab+and+NI+Version+Compatibility|Matlab Compatibility matrix]]
   * [[Retune scan mirror controllers]]   * [[Retune scan mirror controllers]]
   * [[Tune Piezo]] e.g to make it as fast as possible for specific objective/step size   * [[Tune Piezo]] e.g to make it as fast as possible for specific objective/step size
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   * [[aaron:projects:excitation_of_alexa_dyes_and_fps|Excitation Wavelengths for dyes and fluorescent proteins]]   * [[aaron:projects:excitation_of_alexa_dyes_and_fps|Excitation Wavelengths for dyes and fluorescent proteins]]
   * [[Change Coolant for Laser]]   * [[Change Coolant for Laser]]
 +  * [[2P Dissections]]
 +  * [[https://www.biorxiv.org/content/10.1101/2024.01.23.576417v1|Packer Multiphoton Testing Protocols]]
 +====== Safety ======
 +
 +See also the general lab [[safety:start|Safety page]]
 +
 +  * [[2P Risk Assessment]]
 +  * Class IV Laser Safety RA {{ :equipment:multiphoton:ra_sutter_movable_objective_multiphoton_microscope.docx |}}
 +  * Standard Operating Procedures {{ :equipment:multiphoton:sutter_movable_objective_microscope_sop_normal_and_alignment_use.pdf |}}
 ====== Maintenance ====== ====== Maintenance ======
   * We have a 3 year extended warranty with Coherent until 18th March 2014 - {{:equipment:multiphoton:coherentmaintenance2011-2014.pdf|details in this pdf}}   * We have a 3 year extended warranty with Coherent until 18th March 2014 - {{:equipment:multiphoton:coherentmaintenance2011-2014.pdf|details in this pdf}}
-  *  Contact at Coherent is Mike Shearer - Email: mike.shearer@coherent.com - Tel: 01353 65 88 54 +  *  Contact at Coherent is Mike Shearer (mike.shearer@coherent.com - Tel: 01353 65 88 54)
  

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