see full list chemical labeling fly stocks

• {myrSNAP}attP40 or {myrSNAP}attP2 better signal in neurites
• CD4-SNAP/CyO;CD4-SNAP/TM6b stock enriched in cell bodies

• CD4-CLIP#1/CyO no myrCLIP available at the moment

• have used single attP40 and attP2 insertions which all worked; could try two insertions

• {myrTMP}attP40/CyO; {myrTMP}attP2 even when using this double insertion, signal is very weak (increase fixation to 40-60 min); only really useful for neuropil labeling

only SNAP and CLIP at the moment; in general, SNAP gives better signal-to-noise

pre

• SytCLIP#4/CyO or SytCLIP#6/TM3 these two P-element insertions have high expression levels (see list)
• SytSNAP#3/CyO or SytSNAP#2/TM3 these two P-element insertions have high expression levels (see list)
• CLIPsyb P-element insertions on 2 and 3, haven't thoroughly compared expression levels
• SNAPsyb P-element insertions on 2 and 3, haven't thoroughly compared expression levels

post

• TLN-CLIP#6/CyO or TLN-CLIP#9/TM3 these two P-element insertions have high expression levels (see list)
• TLN-SNAP#4/CyO or TLM-SNAP#5/TM3 these two P-element insertions have high expression levels (see list)

• brp-SNAP/CyO knock-in based on MiMIC insertion
• dlg-SNAP knock-in based on MiMIC insertion
• dlg1-SNAP product of P-element swap of protein trap line; weaker than the knock-in line
• dlg1-CLIP product of P-element swap of protein trap line

• labeling speed: SNAP > Halo > CLIP > TMP
• signal-to-noise: Halo > SNAP > CLIP > TMP
• signal intensity (with best available subtrate): SNAP,Halo > CLIP > TMP

take into consideration e.g. when using one tag for single-cell labeling and a second one for neuropil labeling