In additional to supplemental data available via the Current Biology website, we are providing two large collections of image data:

  1. 3Gb of key image data (available on the Web)
  2. >1Tb of comprehensive image data including all raw and processed confocal stacks (available by external hard drive)

Web Supplement

You can access the web supplemental data here:

http://flybrain.mrc-lmb.cam.ac.uk/CacheroOstrovsky/Data/

The directory hierarchy that you will see includes:

  • Standard Template Brains and Ventral Nerve Cords
    • Brain
    • VNC
  • Single full sized image stacks of each fruitless neuroblast clone
    • Brain
      • Female
      • Male
    • VNC
      • Male
      • Female
  • Resampled image stacks of each clone (suitable for use with Amira)
    • Brain
      • Female
      • Male
    • VNC
      • Male
      • Female
  • Mask files used to extract clones from registered images
    • Brain
    • VNC
  • Registration files used for Deformation Based Morphometry
    • femaleControl
    • fruMFemales
    • maleControl
    • traFemales
    • traMales
  • Doublesex/fruitless double immunolabelled stacks
    • female
    • male

Hard drive Supplement

This includes all raw Brain and VNC data used in our study (1.05 Tb).

There are Brain and VNC top level directories each containing the following :

  • RawLSMs : MARCM Confocal stacks from our Zeiss LSM 710 (984,364 LSM files)
  • Registrations : Registration output folders including settings and logs (737,299 CMTK .list folders)
  • SuccessfullyRegistered : Tissues after registration - brains that did not register well are excluded (1533, 724 PIC files)
  • ExtractedImages : Masked images generated for each clone present in each image (908, 241 NRRD files)
  • TwinSpotBrains: Brains labelled with twin spot MARCM so that clones are either in red or green but not both (270,0 LSM files)
  • A csv spreadsheet ExportedImageRecords.txt which includes additional information about the images used

(numbers in parentheses indicate the number of Brain and VNC images respectively of each type)


Log In