Recommended fly stocks for chemical labeling
membrane labeling
SNAP
CLIP
Halo
TMP
synaptic labeling
only SNAP and CLIP at the moment; in general, SNAP gives better signal-to-noise
pre
SytCLIP#4/CyO or SytCLIP#6/TM3 these two P-element insertions have high expression levels (see list)
SytSNAP#3/CyO or SytSNAP#2/TM3 these two P-element insertions have high expression levels (see list)
CLIPsyb P-element insertions on 2 and 3, haven't thoroughly compared expression levels
SNAPsyb P-element insertions on 2 and 3, haven't thoroughly compared expression levels
post
TLN-CLIP#6/CyO or TLN-CLIP#9/TM3 these two P-element insertions have high expression levels (see list)
TLN-SNAP#4/CyO or TLM-SNAP#5/TM3 these two P-element insertions have high expression levels (see list)
neuropil labeling
brp-SNAP/CyO knock-in based on MiMIC insertion
dlg-SNAP knock-in based on MiMIC insertion
dlg1-SNAP product of P-element swap of protein trap line; weaker than the knock-in line
dlg1-CLIP product of P-element swap of protein trap line
labeling speed: SNAP > Halo > CLIP > TMP
signal-to-noise: Halo > SNAP > CLIP > TMP
signal intensity (with best available subtrate): SNAP,Halo > CLIP > TMP
take into consideration e.g. when using one tag for single-cell labeling and a second one for neuropil labeling