=====Recommended fly stocks for chemical labeling=====

see full list [[here|chemical labeling fly stocks]]

===membrane labeling===

==SNAP==
  * {myrSNAP}attP40 or {myrSNAP}attP2 //better signal in neurites//
  * CD4-SNAP/CyO;CD4-SNAP/TM6b stock //enriched in cell bodies//
==CLIP==
  * CD4-CLIP#1/CyO //no myrCLIP available at the moment//
==Halo==
  * have used single attP40 and attP2 insertions which all worked; could try two insertions 
==TMP==
  * {myrTMP}attP40/CyO; {myrTMP}attP2 //even when using this double insertion, signal is very weak (increase fixation to 40-60 min); only really useful for neuropil labeling// 

===synaptic labeling===
only SNAP and CLIP at the moment; in general, SNAP gives better signal-to-noise

**pre**
  * SytCLIP#4/CyO or SytCLIP#6/TM3 //these two P-element insertions have high expression levels (see list)//
  * SytSNAP#3/CyO or SytSNAP#2/TM3 //these two P-element insertions have high expression levels (see list)//
  * CLIPsyb //P-element insertions on 2 and 3, haven't thoroughly compared expression levels//
  * SNAPsyb //P-element insertions on 2 and 3, haven't thoroughly compared expression levels//
**post**
  * TLN-CLIP#6/CyO or TLN-CLIP#9/TM3 //these two P-element insertions have high expression levels (see list)//
  * TLN-SNAP#4/CyO or TLM-SNAP#5/TM3 //these two P-element insertions have high expression levels (see list)//

===neuropil labeling===
  * brp-SNAP/CyO //knock-in based on MiMIC insertion//
  * dlg-SNAP //knock-in based on MiMIC insertion//
  * dlg1-SNAP //product of P-element swap of protein trap line; weaker than the knock-in line//
  * dlg1-CLIP //product of P-element swap of protein trap line//

=====General Performance of tags=====

  * labeling speed: SNAP > Halo > CLIP > TMP
  * signal-to-noise: Halo > SNAP > CLIP > TMP
  * signal intensity (with best available subtrate): SNAP,Halo > CLIP > TMP 

take into consideration e.g. when using one tag for single-cell labeling and a second one for neuropil labeling