D. sechellia template brains were constructed by Richard Benton and Greg Jefferis using the CMTK registration toolkit essentially as described in Cachero et al. 2010. The [[https://github.com/jefferislab/MakeAverageBrain|MakeAverageBrain]] script collections were used at commit [[https://github.com/jefferislab/MakeAverageBrain/commit/5c411ecb2e9d971e1cefd327c4504c9bc0b2a36b|5c411ecb2e9d971e1cefd327c4504c9bc0b2a36b]]. The template brains have been uploaded to Zenodo and therefore have a DOI associated with a specific version. * [[https://doi.org/10.5281/zenodo.2562141|DsecF]] * [[https://doi.org/10.5281/zenodo.2562640|DsecM]] Bridging registrations were constructed essentially as described in Manton et al 2014. This made use of the [[https://github.com/jefferis/fiji-cmtk-gui|Fiji CMTK plugin]]. The resultant registrations are available for download at [[https://github.com/jefferislab/DrosophilidBridgingRegistrations]]. They can most easily be used for transforming image data or 3D coordinates. Transforming coordinates, neuron tracings and surface models is most easily achieved using the [[http://jefferis.github.io/nat/|nat]] toolbox and in particular the [[http://jefferislab.github.io/nat.flybrains/|nat.flybrains]] which bundles support for these Dsec template brains.