We used the following command lines to run ''munger.pl'' for both brains and VNCs: munger.pl -b /path/to/cmtk/binaries -vawr 010203 -T 4 -X 26 -C 8 -G 80 -R 4 \ -A '--accuracy 0.4' -W '--accuracy 0.4' -s "refbrain/IS2.PIC" "images" # identical except for reference image munger.pl -b /path/to/cmtk/binaries -awr 010203 -T 4 -X 26 -C 8 -G 80 -R 4 \ -A '--accuracy 0.4' -W '--accuracy 0.4' -s "refbrain/VNCIS1.PIC" "images" This assumes that munger is called from inside a registration folder with the following structure * **''RegistrationFolder''** (or whatever name you choose) * **''images''** (contains images to register in nrrd (preferred) or PIC format) * **''refbrain''** (contains reference brain) Note that /path/to/cmtk/binaries should only required if they are installed in a directory that is not in your path. On a mac, the default CMTK install will put them in ''/opt/local/bin/''. For the registration GUI, we would just need the following options as the others are set interactively through dialog boxes: -awr 010203 -T 4 -X 26 -C 8 -G 80 -R 4 -A '--accuracy 0.4' -W '--accuracy 0.4' See [[warping_manual:custom_command_lines_for_munger.pl]] and [[warping_manual:registration_parameters]] for further details.