In additional to supplemental data available via the Current Biology website, we are providing two large collections of image data: - 3Gb of key image data (available on the Web) - >1Tb of comprehensive image data including all raw and processed confocal stacks (available by external hard drive) ====== Web Supplement ====== You can access the web supplemental data here: http://flybrain.mrc-lmb.cam.ac.uk/CacheroOstrovsky/Data/ The directory hierarchy that you will see includes: * Standard Template Brains and Ventral Nerve Cords * Brain * VNC * Single full sized image stacks of each fruitless neuroblast clone * Brain * Female * Male * VNC * Male * Female * Resampled image stacks of each clone (suitable for use with Amira) * Brain * Female * Male * VNC * Male * Female * Mask files used to extract clones from registered images * Brain * VNC * Registration files used for Deformation Based Morphometry * femaleControl * fruMFemales * maleControl * traFemales * traMales * Doublesex/fruitless double immunolabelled stacks * female * male ====== Hard drive Supplement ====== This includes all raw Brain and VNC data used in our study (1.05 Tb). There are **Brain** and **VNC** top level directories each containing the following : * **RawLSMs** : MARCM Confocal stacks from our Zeiss LSM 710 //(984,364 LSM files)// * **Registrations** : Registration output folders including settings and logs //(737,299 CMTK .list folders)// * **SuccessfullyRegistered** : Tissues after registration - brains that did not register well are excluded// (1533, 724 PIC files)// * **ExtractedImages** : Masked images generated for each clone present in each image //(908, 241 NRRD files)// * **TwinSpotBrains**: Brains labelled with twin spot MARCM so that clones are either in red or green but not both //(270,0 LSM files)// * A csv spreadsheet **ExportedImageRecords.txt** which includes additional information about the images used //(numbers in parentheses indicate the number of Brain and VNC images respectively of each type)//