====== Parts ====== * There is a also a useful [[http://research.janelia.org/labs/display/ephus/ScanImage+Supported+Microscope+Hardware|supported parts]] list for ScanImage software ===== Laser ===== Coherent Chameleon Vision (A, below). Specs are {{:alex:projects:multiphoton:chameleonvision_ds_final.pdf|here}} ===== Core Microscope ===== Sutter [[http://www.sutter.com/MICROSCOPES/mom.html|MOM]] (Moveable Objective Microscope). {{:alex:projects:multiphoton:2pscopeaaron.png|}} * Fluorescence path * Point Grey [[http://www.ptgrey.com/products/grasshopper/grasshopper_firewire_camera.asp|Grasshopper]] CCD camera (A) * Olympus tube lens (B) * Olympus epi-fluorescence path (from BX51) (C) * Mirrors of MOM core (L) * Objective lens (variable) (E) * Two-photon detection path * Objective lens (E) * Dichroic mirror in MOM core (L) * Hamamatsu PMTs (and collection optics) (F) * Hamamatsu pre-amps (G) * Other * PMT Power supply (H) * iMac (O) * Power meters (J) * Power sensor (K) * Stage (D) * Cage light (M) * Cage (N) * Thorlabs cubes (for controlling half-wave plate, fluorescence LED, and shutter (I) * Odour presentation manifold (P) * Aaron's junk (Q) ==== Scan System ==== Fast scan 6mm mirrors (Cambridge 6215) and scanner driver electronics (includes two Cambridge Technologies 671XX-HP Micromax scanner driver boards) ==== PMTs ==== Hamamatsu H7422 P40-MOD unprotected GAsP PMTs (Hamamatsu part number, H10770PB-40 SEL) and Hamamatsu C3719 pre amps. The power supply is labeled "Sutter PS-2" but it looks suspiciously like two Hamamatsu model C3830 supplies piled into a Sutter box. I can't find any info on the PS-2, but the Hamatsu specs are [[http://www.google.co.uk/url?sa=t&source=web&cd=1&ved=0CBcQFjAA&url=http%3A%2F%2Fwww.sales.hamamatsu.com%2Fassets%2Fpdf%2Fparts_C%2FC3830-22.pdf&rct=j&q=C3830-22.pdf&ei=8e2aTYrpCtSahQf04vDXBg&usg=AFQjCNGDNivxZS6CuiCZA-Scu2JU-hqQrA&sig2=Ux5G2eGF8ECaxZyhX-JLzw&cad=rja|here]] or {{:alex:projects:multiphoton:c3830-22.pdf|here.}} This is just a guess on my part, so if you are really fussed about these specs, it would probably be best to contact Sutter. ===== Other Parts ===== {{:alex:projects:multiphoton:beampathmod.png|}} * Filters/dichroic for detection * Chroma * Table * Newport Smart Table UT2 with IQ Damping Technology * Attenuation * Thorlabs (WPH05M) rotating half-wave plate (B) and fixed (GL10-B) Glan-Laser polariser (D) to control beam intensity * Non-polarising (Thorlabs CM1-BS014) 50:50 beam splitter (F) to divide the beam * Variable (Thorlabs BE02-05-B) beam expander (I) to control objective (over/under) filling * Power measurement * J beam sampler (reflected portion is angle dependent - currently around 3%) * L Power meter * note that you need to re-calibrate the linear relationship between power meter reading and power at the objective back aperture if you * knock/realign J * put telescope in * Shutters * Thorlabs SH05 (G) This shutter is normally opened by scanImage during a run. Additionally, it is connected to an interlock on the shroud door, forced closed when the door is open. * Beam steering on table * Thorlabs mirrors (E, H) * Beam trap * Thorlabs BT610/M (C, K) * PCs for control * Lenovo Thinkstation with NI DAQ board (NI 6110E) for ScanImage operation * iMac with Micromanager ===== Objectives ===== * [[https://www.micro-shop.zeiss.com/?s=393578527f2544&l=en&p=uk&f=o&a=v&m=s&id=421452-9900-000|Zeiss 20x]] 1.0 NA M27 (70 mm parfocal, 75 also avail w more DIC) * [[http://www.olympusamerica.com/seg_section/uis2/seg_uis2_xlumplflnw_20xw.asp#|Olympus 20x]] 1.0 NA 25 diam * [[http://www.olympusamerica.com/seg_section/uis2/seg_uis2_lumplfln_40xw.asp|Olympus 40x]] 0.8 NA (RMS mount) * Olympus 25x 1.05 NA (? mount) * [[http://www.nikoninstruments.eu/en_GB/Products/Optics-Objectives/Apochromat-Objectives/Lambda-S-Objective-Series/%28specifications%29|Nikon 25x water 1.1 NA with correction collar]] ====== Software ====== * Svoboda [[http://research.janelia.org/wiki/display/ephus/ScanImage%2C+Ephus%2C+and+other+DAQ+software|ScanImage and Ephus]] ====== Filters for PMTs ====== Alex set up the filter cube for the PMTs as below: RED CHANNEL *-------------* | HQ605/75 \/ | *---*-------------* G | |\* R | E | \* E | T | \* E | 5 | \* N | 2 | \* | 5 | \* C | / | \* H | 7 | \* A | 0 | \* N | | \* N | | \* E | > | \* L | | \* *---* The filters are by Chroma and the arrows point towards the coated side.